pcDNA3-Myc-ASC | Addgene 73952 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3-Myc-ASC

catalog :

73952

citations: 3

Reference
Xing J, Zhou X, Fang M, Zhang E, Minze L, Zhang Z. DHX15 is required to control RNA virus-induced intestinal inflammation. Cell Rep. 2021;35:109205 pubmed publisher
LaRock D, Sands J, Ettouati E, Richard M, Bushway P, Adler E, et al. Inflammasome inhibition blocks cardiac glycoside cell toxicity. J Biol Chem. 2019;294:12846-12854 pubmed publisher
Bryan N, Dorfleutner A, Rojanasakul Y, Stehlik C. Activation of inflammasomes requires intracellular redistribution of the apoptotic speck-like protein containing a caspase recruitment domain. J Immunol. 2009;182:3173-82 pubmed publisher

product information

Catalog Number :

73952

Product Name :

pcDNA3-Myc-ASC

article :

doi	10.4049/jimmunol.0802367
id	17871
pubmed_id	19234215

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

A c-myc tag and a modified MCS were inserted as indicated in the attached plasmid map.

backbone_origin

backbone_size

5459

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Express c-Myc-tagged human ASC in mammalian cells

origin :

pi :

alt_names

ASC

TMS-1

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3	SP6 promoter (ATTTAGGTGACACTATAG)
sequencing_primer_5	T7 promoter (TAATACGACTCACTATAGGG)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ASC, CARD5, TMS, TMS-1, TMS1
gene	PYCARD
id	29108

genbank_ids

NM_013258.4

mutation

name

PYD and CARD domain containing, PYCARD

shRNA_sequence

size

636

species

9606

Homo sapiens