This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-15b_LC3B
catalog :
73949
citations: 5
Reference
Stykel M, Humphries K, Kamski Hennekam E, Buchner Duby B, Porte Trachsel N, Ryan T, et al. α-Synuclein mutation impairs processing of endomembrane compartments and promotes exocytosis and seeding of α-synuclein pathology. Cell Rep. 2021;35:109099 pubmed publisher
Usategui Martín R, Gestoso Uzal N, Calero Paniagua I, de Pereda J, del Pino Montes J, Gonzalez Sarmiento R. A mutation in p62 protein (p. R321C), associated to Paget's disease of bone, causes a blockade of autophagy and an activation of NF-kB pathway. Bone. 2020;133:115265 pubmed publisher
Abdollahzadeh I, Hendriks J, Sanwald J, Simons I, Hoffmann S, Weiergräber O, et al. Autophagy-Related Proteins GABARAP and LC3B Label Structures of Similar Size but Different Shape in Super-Resolution Imaging. Molecules. 2019;24: pubmed publisher
Ryan T, Bamm V, Stykel M, Coackley C, Humphries K, Jamieson Williams R, et al. Cardiolipin exposure on the outer mitochondrial membrane modulates α-synuclein. Nat Commun. 2018;9:817 pubmed publisher
Ma P, Schwarten M, Schneider L, Boeske A, Henke N, Lisak D, et al. Interaction of Bcl-2 with the autophagy-related GABAA receptor-associated protein (GABARAP): biophysical characterization and functional implications. J Biol Chem. 2013;288:37204-15 pubmed publisher
product information
Catalog Number :
73949
Product Name :
pET-15b_LC3B
article :
doi10.1074/jbc.M113.528067
id16551
pubmed_id24240096
bacterial resistance :
Ampicillin
cloning :
backbonepET-15b
backbone_mutation
backbone_originNovagen
backbone_size5700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
For recombinant expression of human LC3B/MAP1LC3B in E. coli
origin :
37
pi :
alt_names
MAP1LC3B
cloning
clone_methodRestriction Enzyme
cloning_site_3Xho I
cloning_site_5BamH I
promoterT7
sequencing_primer_3
sequencing_primer_5T7 TAATACGACTCACTATAGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
geneMAP1LC3B
id81631
genbank_ids
mutation
nameLC3B
shRNA_sequence
size372
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis6
resistance markers :
2390
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA