This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET151-GFP-PP-RPN2 (932-953)
catalog :
73745
citations: 1
Reference
VanderLinden R, Hemmis C, Yao T, Robinson H, Hill C. Structure and energetics of pairwise interactions between proteasome subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment mechanism. J Biol Chem. 2017;292:9493-9504 pubmed publisher
product information
Catalog Number :
73745
Product Name :
pET151-GFP-PP-RPN2 (932-953)
article :
doi10.1074/jbc.M117.785287
id25622
pubmed_id28442575
bacterial resistance :
Ampicillin
cloning :
backbonepET151-D-TOPO
backbone_mutation
backbone_origin
backbone_size5800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Residues 932 and 933 of RPN2 are GP, which are also the C-terminal residues of the PreScission Protease recognition sequence that directly precedes RPN2 in our construct. For this reason, the inserted sequence of RPN2 corresponds to residues 934-953, which will result in the expression of the RPN2 932-953 peptide sequence.
growth strain :
Expression of GFP-PP-RPN2 (932-953) fusion in E.coli
origin :
37
pi :
alt_names
S1
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRIBIIR, RPN-II, RPNII, SWP1
geneRPN2
id6185
genbank_ids
NM_001191037.1
mutationConstruct possesses only residues 932-953 of RPN2
nameRPN2
shRNA_sequence
size45
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP
locationN terminal on backbone
tag6x His
resistance markers :
2231
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA