This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET24a-AlkB deltaN11
catalog :
73622
citations: 3
Reference
Warren J, Salinas Giegé T, Triant D, Taylor D, Drouard L, Sloan D. Rapid Shifts in Mitochondrial tRNA Import in a Plant Lineage with Extensive Mitochondrial tRNA Gene Loss. Mol Biol Evol. 2021;38:5735-5751 pubmed publisher
Warren J, Salinas Giegé T, Hummel G, Coots N, Svendsen J, Brown K, et al. Combining tRNA sequencing methods to characterize plant tRNA expression and post-transcriptional modification. RNA Biol. 2021;18:64-78 pubmed publisher
Frick L, Delaney J, Wong C, Drennan C, Essigmann J. Alleviation of 1,N6-ethanoadenine genotoxicity by the Escherichia coli adaptive response protein AlkB. Proc Natl Acad Sci U S A. 2007;104:755-60 pubmed
product information
Catalog Number :
73622
Product Name :
pET24a-AlkB deltaN11
article :
doi10.1073/pnas.0607377104
id16370
pubmed_id17213319
bacterial resistance :
Kanamycin
cloning :
backbonepET24a
backbone_mutation
backbone_originNovagen
backbone_size5310
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7 terminal
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb2212, ECK2204, aidD
genealkB
id946708
genbank_ids
mutationdeletion of first 11 AA
nameAlkB
shRNA_sequence
size
species
32644
Other
E. coli
tags
locationC terminal on backbone
tagHis
resistance markers :
2728
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA