pET15b/bPanK | Addgene 73619 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET15b/bPanK

catalog :

73619

citations: 2

Reference
Yang L, Lin K, Zhu L, Wang H, Teng S, Huang L, et al. Long non-coding RNA DARS-AS1 promotes tumor progression by directly suppressing PACT-mediated cellular stress. Commun Biol. 2022;5:822 pubmed publisher
Calder R, Williams R, Ramaswamy G, Rock C, Campbell E, Unkles S, et al. Cloning and characterization of a eukaryotic pantothenate kinase gene (panK) from Aspergillus nidulans. J Biol Chem. 1999;274:2014-20 pubmed

product information

Catalog Number :

73619

Product Name :

pET15b/bPanK

article :

doi
id	16573
pubmed_id	9890959

bacterial resistance :

Ampicillin

cloning :

backbone

pET15b

backbone_mutation

backbone_origin

backbone_size

5708

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Amplified gene from pWS7-13-2 [described in WJ Song & S Jackowski (1992) J. Bacteriol. 174: 6411 (PMID 1328157)]. Cloning described as blunt ligation of blunted panK fragment (AcyI/PstI) into blunted BamHI cut pET15b. The restriction sites described as used for cloning (NdeI-BamHI) are unique in the plasmid.

growth strain :

Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

origin :

pi :

alt_names

CoaA

panK

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	NdeI
promoter	T7
sequencing_primer_3	T7 terminator
sequencing_primer_5	T7 promoter
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	b3974, ECK3966, JW3942, panK, rts, ts-9
gene	coaA
id	948479

genbank_ids

WP_001305325.1

mutation

relative to MG1655 sequence, changes glutamate-5 to aspartate

name

pantothenate kinase

shRNA_sequence

size

species

32644

Other

Escherichia coli