hTRAFD1-pCMV14-3xFlag | Addgene 73587 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

hTRAFD1-pCMV14-3xFlag

catalog :

73587

citations: 1

Reference
Witwicka H, Jia H, Kutikov A, Reyes Gutiérrez P, Li X, Odgren P. TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast Acidification and Resorption. PLoS ONE. 2015;10:e0127537 pubmed publisher

product information

Catalog Number :

73587

Product Name :

hTRAFD1-pCMV14-3xFlag

article :

doi	10.1371/journal.pone.0127537
id	15318
pubmed_id	25992615

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV-14-3xFlag

backbone_mutation

backbone_origin

Sigma Aldrich

backbone_size

6270

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Express human TRAFD1 (FLN29) in mammalian cells

origin :

pi :

alt_names

FLN29

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	CMV fwd primer
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	FLN29
gene	TRAFD1
id	10906

genbank_ids

mutation

The original vector hTRAFD1-mCMV14-Flag was a gift from Dr. Akihiko Yoshimura, Keio University, Tokyo, Japan. We removed the stop codon from TRAFD1 (prior to Flag tag) so we could pull down Trafd1 using Flag beads. Flag doesn't have start codon

name

TRAFD1

shRNA_sequence

size

1749

species

9606

Homo sapiens