This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCZ886
catalog :
73584
citations: 1
Reference
Noma K, Jin Y. Optogenetic mutagenesis in Caenorhabditis elegans. Nat Commun. 2015;6:8868 pubmed publisher
product information
Catalog Number :
73584
Product Name :
pCZ886
article :
doi10.1038/ncomms9868
id16562
pubmed_id26632265
bacterial resistance :
Ampicillin
cloning :
backbonepCFJ150
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
Other
For MosSCI insertion
growth strain :
Pmex-5-HIS-72-miniSOG-3'UTR(tbb-2) for optogenetic mutagenesis
origin :
37
pi :
eleganstags
locationC terminal on insert
tagminiSOG

Cloningcloning_site_3
cloning_site
_5promotertd>Pmex-5sequenci
ng_primer_3GTACCAGAGCTCACCTAGG
caggaacagctatgacccaatgagacttttttcttggcg
gcsequencing_primer_5
GCACCGTACGTCTCGAGtgtaaaacgacgg
ccagtgtacgactcactatagggcgaattg>site_3_destroyed
site_5_destroyed<
/td>entr
ez_gene
<
tr>
>
aliasesCELE_Y49E10.6d>
genehis-72
id176660
r>genbank_ids>mutation>nameHIS-72s
hRNA_sequence>size467spec
ies
<
td>6239
Caenorhabditis
alt_names
Histone 72, H3 histone
cloning
clone_methodGibson
plasmid copy :
The backbone is pCFJ150, made in the lab of Erik M. Jorgensen, University of Utah.
resistance markers :
2411
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA