This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mCherry-CAD
catalog :
73566
citations: 3
Reference
Jazbec V, Jerala R, Bencina M. Proteolytically Activated CRAC Effectors through Designed Intramolecular Inhibition. ACS Synth Biol. 2022;: pubmed publisher
Zheng S, Ma G, He L, Zhang T, Li J, Yuan X, et al. Identification of molecular determinants that govern distinct STIM2 activation dynamics. PLoS Biol. 2018;16:e2006898 pubmed publisher
Dynes J, Amcheslavsky A, Cahalan M. Genetically targeted single-channel optical recording reveals multiple Orai1 gating states and oscillations in calcium influx. Proc Natl Acad Sci U S A. 2016;113:440-5 pubmed publisher
product information
Catalog Number :
73566
Product Name :
mCherry-CAD
article :
doi10.1073/pnas.1523410113
id16559
pubmed_id26712003
bacterial resistance :
Kanamycin
cloning :
backbonepmChery-C1
backbone_mutation
backbone_originClontech
backbone_size3954
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
CAD corresponds to amino acids 342-448 of human STIM1.
growth strain :
Soluble red fluorescent activator of Orai1 channels
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH1
cloning_site_5AgeI
promoterCMV Immediate Early
sequencing_primer_3ACATTGATGAGTTTGGAC
sequencing_primer_5TGACGTCAATGGGAGTTTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesD11S4896E, GOK, IMD10, STRMK, TAM, TAM1
geneSTIM1
id6786
genbank_ids
NM_003156
mutation
namemCherry-CAD (STIM1 342-448)
shRNA_sequence
size1092
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagmCherry
locationC terminal on insert
tag6x His
resistance markers :
2747
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA