G-GECO1.2-Orai1Y80E | Addgene 73565 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

G-GECO1.2-Orai1Y80E

catalog :

73565

citations: 2

Reference
Cantonero C, Camello P, Salido G, Rosado J, Redondo P. TMEM97 facilitates the activation of SOCE by downregulating the association of cholesterol to Orai1 in MDA-MB-231 cells. Biochim Biophys Acta Mol Cell Biol Lipids. 2021;1866:158906 pubmed publisher
Dynes J, Amcheslavsky A, Cahalan M. Genetically targeted single-channel optical recording reveals multiple Orai1 gating states and oscillations in calcium influx. Proc Natl Acad Sci U S A. 2016;113:440-5 pubmed publisher

product information

Catalog Number :

73565

Product Name :

G-GECO1.2-Orai1Y80E

article :

doi	10.1073/pnas.1523410113
id	16559
pubmed_id	26712003

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C2

backbone_mutation

The EGFP gene has been replaced with the genetically encoded calcium indicator G-GECO1.2

backbone_origin

Clontech

backbone_size

3954

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The fusion protein in this plasmid contains the genetically encoded calcium indicator G-GECO1.2 from the plasmid CMV-G-GECO1.2 (Addgene plasmid # 32446) provided by Robert Campbell. Note that the hOrai1 gene isolated in the Cahalan lab contains a silent mutation/polymorphism (T798C; aa T266).

growth strain :

Green fluorescent reporter of mutant Orai1Y80E-associated calcium influx

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	AgeI
promoter	CMV Immediate Early
sequencing_primer_3	ACATTGATGAGTTTGGAC
sequencing_primer_5	TGACGTCAATGGGAGTTTG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CRACM1, IMD9, ORAT1, TAM2, TMEM142A
gene	ORAI1
id	84876

genbank_ids

NM_032790.3

JN258415.1

mutation

changed tyrosine 80 of Orai1 to glutamate

name

G-GECO1.2-Orai1Y80E

shRNA_sequence

size

2184

species

9606

Homo sapiens

100

Synthetic