This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Orai1-GCaMP6f
catalog :
73564
citations: 5
Reference
Kodakandla G, West S, Wang Q, Tewari R, Zhu M, Akimzhanov A, et al. Dynamic S-acylation of the ER-resident protein stromal interaction molecule 1 (STIM1) is required for store-operated Ca2+ entry. J Biol Chem. 2022;298:102303 pubmed publisher
Jazbec V, Jerala R, Bencina M. Proteolytically Activated CRAC Effectors through Designed Intramolecular Inhibition. ACS Synth Biol. 2022;: pubmed publisher
West S, Kodakandla G, Wang Q, Tewari R, Zhu M, Boehning D, et al. S-acylation of Orai1 regulates store-operated Ca2+ entry. J Cell Sci. 2022;135: pubmed publisher
Maneshi M, Toth A, Ishii T, Hori K, Tsujikawa S, Shum A, et al. Orai1 Channels Are Essential for Amplification of Glutamate-Evoked Ca2+ Signals in Dendritic Spines to Regulate Working and Associative Memory. Cell Rep. 2020;33:108464 pubmed publisher
Dynes J, Amcheslavsky A, Cahalan M. Genetically targeted single-channel optical recording reveals multiple Orai1 gating states and oscillations in calcium influx. Proc Natl Acad Sci U S A. 2016;113:440-5 pubmed publisher
product information
Catalog Number :
73564
Product Name :
Orai1-GCaMP6f
article :
doi10.1073/pnas.1523410113
id16559
pubmed_id26712003
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutationThe EGFP gene has been replaced with the genetically encoded calcium indicator GCaMP6f
backbone_originClontech
backbone_size3958
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The fusion protein in this plasmid contains the genetically encoded calcium indicator GCaMP6f from the plasmid pGP-CMV-GCaMP6f (Addgene plasmid # 40755) provided by Douglas Kim. Note that the hOrai1 gene isolated in the Cahalan lab contains a silent mutation/polymorphism (T798C; aa T266).
growth strain :
Green fluorescent reporter of Orai1-associated calcium influx
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BglII
promoterCMV Immediate Early
sequencing_primer_3ACATTGATGAGTTTGGAC
sequencing_primer_5TGACGTCAATGGGAGTTTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCRACM1, IMD9, ORAT1, TAM2, TMEM142A
geneORAI1
id84876
genbank_ids
NM_032790.3
mutation
nameOrai1-GCaMP6f
shRNA_sequence
size2208
species
9606
Homo sapiens
100
Synthetic
tags
locationC terminal on insert
tagGCaMP6f
resistance markers :
2747
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA