G-GECO1.2-Orai1 | Addgene 73562 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

G-GECO1.2-Orai1

catalog :

73562

citations: 5

Reference
Sanchez Collado J, Lopez J, Jardin I, Berna Erro A, Camello P, Cantonero C, et al. Orai1α, but not Orai1β, co-localizes with TRPC1 and is required for its plasma membrane location and activation in HeLa cells. Cell Mol Life Sci. 2022;79:33 pubmed publisher
Löhndorf A, Hosang L, Dohle W, Odoardi F, Waschkowski S, Rosche A, et al. 2-Methoxyestradiol and its derivatives inhibit store-operated Ca2+ entry in T cells: identification of a new and potent inhibitor. Biochim Biophys Acta Mol Cell Res. 2021;:118988 pubmed publisher
Sanchez Collado J, Lopez J, Jardin I, Camello P, Falcon D, Regodón S, et al. Adenylyl Cyclase Type 8 Overexpression Impairs Phosphorylation-Dependent Orai1 Inactivation and Promotes Migration in MDA-MB-231 Breast Cancer Cells. Cancers (Basel). 2019;11: pubmed publisher
Diercks B, Werner R, Weidemüller P, Czarniak F, Hernandez L, Lehmann C, et al. ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation. Sci Signal. 2018;11: pubmed publisher
Dynes J, Amcheslavsky A, Cahalan M. Genetically targeted single-channel optical recording reveals multiple Orai1 gating states and oscillations in calcium influx. Proc Natl Acad Sci U S A. 2016;113:440-5 pubmed publisher

product information

Catalog Number :

73562

Product Name :

G-GECO1.2-Orai1

article :

doi	10.1073/pnas.1523410113
id	16559
pubmed_id	26712003

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C2

backbone_mutation

The EGFP gene has been replaced with the genetically encoded calcium indicator G-GECO1.2

backbone_origin

Clontech

backbone_size

3954

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The fusion protein in this plasmid contains the genetically encoded calcium indicator G-GECO1.2 from the plasmid CMV-G-GECO1.2 (Addgene plasmid # 32446) provided by Robert Campbell. Note that the hOrai1 gene isolated in the Cahalan lab contains a silent mutation/polymorphism (T798C; aa T266).

growth strain :

Green fluorescent reporter of Orai1-associated calcium influx

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	AgeI
promoter	CMV Immediate Early
sequencing_primer_3	ACATTGATGAGTTTGGAC
sequencing_primer_5	TGACGTCAATGGGAGTTTG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CRACM1, IMD9, ORAT1, TAM2, TMEM142A
gene	ORAI1
id	84876

genbank_ids

NM_032790.3

JN258415.1

mutation

name

G-GECO1.2-Orai1

shRNA_sequence

size

2184

species

9606

Homo sapiens

100

Synthetic