pCW57.1 _3xHA-Ago2_CATmut | Addgene 73540 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCW57.1 _3xHA-Ago2_CATmut

catalog :

73540

citations: 1

Reference
Ngondo R, Cirera Salinas D, Yu J, Wischnewski H, Bodak M, Vandormael Pournin S, et al. Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells. Stem Cell Reports. 2018;10:461-476 pubmed publisher

product information

Catalog Number :

73540

Product Name :

pCW57.1 _3xHA-Ago2_CATmut

article :

doi	10.1016/j.stemcr.2017.12.023
id	28192836
pubmed_id	29396181

bacterial resistance :

Ampicillin

cloning :

backbone

pCW57.1

backbone_mutation

backbone_origin

David Root (Addgene plasmid # 41393)

backbone_size

9354

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Vector linearized using NheI/BamH1 sites. Insert cloned by recombination using In-Fusion cloning. Note that this plasmid grows slowly. Liquid cultures may need to be incubated up to two days for substantial growth.

growth strain :

Inducible lentiviral expression of Ago2_CATmut

growth temp :

Note that this plasmid grows slowly. Liquid cultures may need to be incubated up to two days for substantial growth.

inserts :

Note that this plasmid grows slowly. Liquid cultures may need to be incubated up to two days for substantial growth.

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	TRE promoter, Tet ON
sequencing_primer_3	GAACGGACGTGAAGAATGTG
sequencing_primer_5	LNCX
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	1110029L17Rik, 2310051F07Rik, Eif2c2, Gerp95, Gm10365, mKIAA4215
gene	Ago2
id	239528

genbank_ids

mutation

Catalytic mutant PIWI domain D670A

name

Ago2

shRNA_sequence

size

species

10090

Mus musculus