This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDNA3-HA-Akt1-K179M
catalog :
73409
citations: 4
Reference
Yu Z, Chen J, Takagi E, Wang F, Saha B, Liu X, et al. Interactions between mTORC2 core subunits Rictor and mSin1 dictate selective and context-dependent phosphorylation of substrate kinases SGK1 and Akt. J Biol Chem. 2022;298:102288 pubmed publisher
Palma M, Leroy C, Salomé Desnoulez S, Werkmeister E, Kong R, Mongy M, et al. A role for AKT1 in nonsense-mediated mRNA decay. Nucleic Acids Res. 2021;49:11022-11037 pubmed publisher
Li T, Wang X, Ju E, da Silva S, Chen L, Zhang X, et al. RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation. Nat Commun. 2021;12:1055 pubmed publisher
Zeng M, van der Donk W, Chen J. Lanthionine synthetase C-like protein 2 (LanCL2) is a novel regulator of Akt. Mol Biol Cell. 2014;25:3954-61 pubmed publisher
product information
Catalog Number :
73409
Product Name :
pCDNA3-HA-Akt1-K179M
article :
doi10.1091/mbc.E14-01-0004
id14043
pubmed_id25273559
bacterial resistance :
Ampicillin
cloning :
backbonepCDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
pCDNA3 HA-Akt1-K179M was subcloned from pLNCX-HA-Akt1-K179M (Addgene plasmid 9007; Ramaswamy et al., 1999).
growth strain :
Expresses kinase-inactive mutant of Akt with HA tag
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
geneAKT1
id207
genbank_ids
mutationchanged K179M to make Kinase inactive mutant
nameAKT1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA tag
resistance markers :
818
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA