This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-2T-FRB-W2027F
catalog :
73341
citations: 1
Reference
Vilella Bach M, Nuzzi P, Fang Y, Chen J. The FKBP12-rapamycin-binding domain is required for FKBP12-rapamycin-associated protein kinase activity and G1 progression. J Biol Chem. 1999;274:4266-72 pubmed
product information
Catalog Number :
73341
Product Name :
pGEX-2T-FRB-W2027F
article :
doi10.1074/jbc.274.7.4266
id3712
pubmed_id9933627
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-2T
backbone_mutation
backbone_originAmersham Pharmacia Biotech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Point mutation Trp 2027 Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR
origin :
37
pi :
alt_names
Mtor, FRAP
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3pGEX-3
sequencing_primer_5pGEX-5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
geneMTOR
id2475
genbank_ids
mutationFRB domain only aa2015-2114; W2027F
nameFKBP12-rapamycin binding (FRB) domain of MTOR
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST-tagged
resistance markers :
818
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA