pcDNA3.1-KozATG-dL5-2XG4S-actin | Addgene 73262 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3.1-KozATG-dL5-2XG4S-actin

catalog :

73262

citations: 1

Reference
Yan Q, Schwartz S, Maji S, Huang F, Szent Gyorgyi C, Lidke D, et al. Localization microscopy using noncovalent fluorogen activation by genetically encoded fluorogen-activating proteins. Chemphyschem. 2014;15:687-695 pubmed publisher

product information

Catalog Number :

73262

Product Name :

pcDNA3.1-KozATG-dL5-2XG4S-actin

article :

doi	10.1002/cphc.201300757
id	9141
pubmed_id	24194371

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

The NdeI site was removed from the CMV promoter

backbone_origin

Invitrogen

backbone_size

5382

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid. http://pathways.mbic.cmu.edu/

growth strain :

Expresses dL5(E52D)-actin fusion within mammalian cells. (MBIC5, dL5**, FAP)

growth temp :

No special instructions.

origin :

pi :

alt_names

dL5-actin

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	BamHI
promoter	CMV
sequencing_primer_3	BGHR
sequencing_primer_5	CMVF
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
gene	ACTB
id	60

genbank_ids

mutation

The dL5** FAP is E50D, L89S, also known as E52D/L91S and NP138

name

KozATG-dL5-2XG4S-actin

shRNA_sequence

size

1950

species

9606

Homo sapiens

100

Synthetic