pET21-10XHis-GST-HRV-Her1-dL5 | Addgene 73216 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET21-10XHis-GST-HRV-Her1-dL5

catalog :

73216

citations: 1

Reference
Wang Y, Telmer C, Schmidt B, Franke J, Ort S, Arndt Jovin D, et al. Fluorogen activating protein-affibody probes: modular, no-wash measurement of epidermal growth factor receptors. Bioconjug Chem. 2015;26:137-44 pubmed publisher

product information

Catalog Number :

73216

Product Name :

pET21-10XHis-GST-HRV-Her1-dL5

article :

doi	10.1021/bc500525b
id	16490
pubmed_id	25490520

bacterial resistance :

Ampicillin

cloning :

backbone

pET21a

backbone_mutation

none

backbone_origin

Novagen

backbone_size

5387

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid. http://pathways.mbic.cmu.edu/

growth strain :

Bacterial expression vector for Z1907-dL5(E52D) affibody fusion protein (AF) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

growth temp :

For expression use a strain that contains T7 RNA polymerase and trxB/gor double mutant such as Rosetta-gami(DE3)

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NdeI and HindIII for Affibody-FAP
promoter	T7
sequencing_primer_3	T7term
sequencing_primer_5	T7 and GSTSeqF
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA
gene	EGFR
id	1956

genbank_ids

mutation

The dL5** FAP is E50D, L89S, also known as E52D/L91S and NP138

name

10XHis-GST-HRV-Her1-dL5

shRNA_sequence

size

1662

species

9606

Homo sapiens

100

Synthetic

32644

Other

Schistosoma japonicum