This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL
catalog :
73209
citations: 2
Reference
Nascimento A, Erustes A, Reckziegel P, Bincoletto C, Ureshino R, Pereira G, et al. α-Synuclein Overexpression Induces Lysosomal Dysfunction and Autophagy Impairment in Human Neuroblastoma SH-SY5Y. Neurochem Res. 2020;45:2749-2761 pubmed publisher
Telmer C, Verma R, Teng H, Andreko S, Law L, Bruchez M. Rapid, specific, no-wash, far-red fluorogen activation in subcellular compartments by targeted fluorogen activating proteins. ACS Chem Biol. 2015;10:1239-46 pubmed publisher
product information
Catalog Number :
73209
Product Name :
pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL
article :
doi10.1021/cb500957k
id16330
pubmed_id25650487
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutationNdeI site was removed from the CMV promoter. XhoI site duplicated for insertion of KDEL coding sequence and STOP codon.
backbone_originInvitrogen
backbone_size5393
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid. http://pathways.mbic.cmu.edu/ Details of mCerulean3 can be found in: Markwardt, M. L., Kremers, G. J., Kraft, C. A., Ray, K., Cranfill, P. J., Wilson, K. A., Day, R. N., Wachter, R. M., Davidson, M. W., and Rizzo, M. A. (2011) An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching. PLoS One 6, e17896.
growth strain :
Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)
growth temp :
No special instructions.
origin :
37
pi :
alt_names
ER
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3BGHR
sequencing_primer_5CMVF
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMRTL, MYCC, bHLHe39, c-Myc
geneMYC
id4609
genbank_ids
mutationThe dL5** FAP is E50D, L89S, also known as E52D/L91S and NP138
namekappa-myc-dL5-2XG4S-mCer3-KDEL
shRNA_sequence
size1674
species
9606
Homo sapiens
100
Synthetic
tags
location
tagThe FAP and mCerulean3 are fused with 2 copies of a G4S linker
location
tagmyc 1020-1049
plasmid copy :
The mCerulean3 is from MA Rizzo, Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
resistance markers :
2743
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA