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product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1-kappa-myc-dL5-2xG4S-TMst
catalog :
73206
citations: 2
Reference
Perkins L, Bruchez M. Fluorogen activating protein toolset for protein trafficking measurements. Traffic. 2020;21:333-348 pubmed publisher
Telmer C, Verma R, Teng H, Andreko S, Law L, Bruchez M. Rapid, specific, no-wash, far-red fluorogen activation in subcellular compartments by targeted fluorogen activating proteins. ACS Chem Biol. 2015;10:1239-46 pubmed publisher
product information
Catalog Number :
73206
Product Name :
pcDNA3.1-kappa-myc-dL5-2xG4S-TMst
article :
doi10.1021/cb500957k
id16330
pubmed_id25650487
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutationThe NdeI site was removed from the CMV promoter. The XhoI in the vector was destroyed. Another was introduced with the insert.
backbone_originInvitrogen
backbone_size5388
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid. http://pathways.mbic.cmu.edu/ For more information about this plasmid, please see the associated publication (PMID: 25650487) as well as He et al. Nat Methods. 2016 Jan 25. doi: 10.1038/nmeth.3735. [Epub ahead of print] PMID: 26808669.
growth strain :
Expresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)
growth temp :
No special instructions.
origin :
37
pi :
alt_names
dL5-TM
Surface
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI/XhoI but another XhoI introduced with the insert
cloning_site_5BamHI
promoterCMV
sequencing_primer_3BGHR
sequencing_primer_5CMVF
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesMRTL, MYCC, bHLHe39, c-Myc
geneMYC
id4609
genbank_ids
mutationThe dL5** FAP is E50D, L89S, also known as E52D/L91S and NP138
namekappa-myc-dL5-2XG4S-TM
shRNA_sequence
size1071
species
9606
Homo sapiens
tags
location
tagThe FAP is fused with 2 copies of a G4S linker
location
tagmyc 1020-1049
resistance markers :
2743
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA