This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3
catalog :
73205
citations: 1
Reference
Telmer C, Verma R, Teng H, Andreko S, Law L, Bruchez M. Rapid, specific, no-wash, far-red fluorogen activation in subcellular compartments by targeted fluorogen activating proteins. ACS Chem Biol. 2015;10:1239-46 pubmed publisher
product information
Catalog Number :
73205
Product Name :
pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3
article :
doi10.1021/cb500957k
id16330
pubmed_id25650487
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutationNdeI site was destroyed by adding tata into the CMV promoter at 487-490
backbone_originInvitrogen
backbone_size5382
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid. http://pathways.mbic.cmu.edu/ For information concerning mCerulean3 please refer to: Markwardt, M. L., Kremers, G. J., Kraft, C. A., Ray, K., Cranfill, P. J., Wilson, K. A., Day, R. N., Wachter, R. M., Davidson, M. W., and Rizzo, M. A. (2011) An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching. PLoSOne 6, e17896. For more information about this plasmid, please see the associated publication (PMID: 25650487) as well as He et al. Nat Methods. 2016 Jan 25. doi: 10.1038/nmeth.3735. [Epub ahead of print] PMID: 26808669.
growth strain :
Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)
growth temp :
No special instructions.
origin :
37
pi :
alt_names
NLS
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3BGHR
sequencing_primer_5CMVF
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMRTL, MYCC, bHLHe39, c-Myc
geneMYC
id4609
genbank_ids
mutationThe dL5** FAP is E50D, L89S, also known as E52D/L91S and NP138
nameNLS-myc-dL5-2XG4S-mCer3
shRNA_sequence
size1623
species
9606
Homo sapiens
100
Synthetic
tags
location
tagThe FAP and mCerulean3 are fused with 2 copies of a G4S linker
location
tagmyc epitope 1020-1049
plasmid copy :
The coding sequence for mCerulean3 was a gift from MA Rizzo, Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
resistance markers :
2743
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA