This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSLC-238
catalog :
73192
citations: 1
Reference
Khetrapal V, Mehershahi K, Rafee S, CHEN S, Lim C, Chen S. A set of powerful negative selection systems for unmodified Enterobacteriaceae. Nucleic Acids Res. 2015;43:e83 pubmed publisher
product information
Catalog Number :
73192
Product Name :
pSLC-238
article :
doi10.1093/nar/gkv248
id16230
pubmed_id25800749
bacterial resistance :
Kanamycin
cloning :
backbonepKD4
backbone_mutationFirst inserted SmaI, AatII, SacII sites and chloramphenicol resistance gene at Afe1 site in pKD4, followed by insertion of PrhaB-chpB at SmaI site using blunt cloning.
backbone_originBarry L. Wanner
backbone_size3267
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
growth temp :
Add 2% glucose
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SmaI
cloning_site_5SmaI
promoterPrhaB
sequencing_primer_3ATTAGCCATGGTCCATATGA
sequencing_primer_5CGGAATCGTTTTCCGGGACG
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesb4225, ECK4221, chpBK, yjfE
genechpB
id948747
genbank_ids
mutation
namePrhaB-chpB
shRNA_sequence
size1315
species
tags
resistance markers :
2716
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA