pSLC-242 | Addgene 73189 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSLC-242

catalog :

73189

citations: 1

Reference
Khetrapal V, Mehershahi K, Rafee S, CHEN S, Lim C, Chen S. A set of powerful negative selection systems for unmodified Enterobacteriaceae. Nucleic Acids Res. 2015;43:e83 pubmed publisher

product information

Catalog Number :

73189

Product Name :

pSLC-242

article :

doi	10.1093/nar/gkv248
id	16230
pubmed_id	25800749

bacterial resistance :

Chloramphenicol

cloning :

backbone

pSLC-217

backbone_mutation

Changed kanamycin resistance gene to chloramphenicol

backbone_origin

Swaine Chen Lab

backbone_size

4549

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

growth temp :

Add 2% glucose

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SacII
cloning_site_5	ApaL1
promoter	chloramphenicol promoter
sequencing_primer_3
sequencing_primer_5	AGTACTGTTGTATTCATTAA
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	pFL129_2
gene	cat
id	2847485

genbank_ids

mutation

name

chloramphenicol

shRNA_sequence

size

1159

species

32644

Other

Although Ampicillin gene is present, it was inactivated while cloning.