This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEF5B-FRT-cilia-APEX
catalog :
73186
citations: 1
Reference
Mick D, Rodrigues R, Leib R, Adams C, Chien A, Gygi S, et al. Proteomics of Primary Cilia by Proximity Labeling. Dev Cell. 2015;35:497-512 pubmed publisher
product information
Catalog Number :
73186
Product Name :
pEF5B-FRT-cilia-APEX
article :
doi10.1016/j.devcel.2015.10.015
id16483
pubmed_id26585297
bacterial resistance :
Ampicillin
cloning :
backbonepEF5B.FRT.DEST
backbone_mutation
backbone_origin
backbone_size6833
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
APEX sequence derived from glycine max according to: Rhee et al., Science, 2013
growth strain :
Expresses cilia-APEX in mammalian cells
origin :
37
pi :
alt_names
NPHP3[1-203]-GFP-APEX
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterEF1alpha
sequencing_primer_3BGH reverse
sequencing_primer_5T7 promotor
site_3_destroyed
site_5_destroyed
entrez_gene
aliases3632410F03Rik, AI550417, C230078J01, D330020E01Rik, pcy
geneNphp3
id74025
genbank_ids
NP_082997
AAA61779.1
mutation
namecilia-APEX
shRNA_sequence
size2253
species
10090
Mus musculus
32644
Other
Glycine max
tags
location
taginternal GFP tag
plasmid copy :
addgene
resistance markers :
735
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA