pZW1-snoVector | Addgene 73174 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pZW1-snoVector

catalog :

73174

citations: 2

Reference
Zhou B, Qi F, Wu F, Nie H, Song Y, Shao L, et al. Endogenous Retrovirus-Derived Long Noncoding RNA Enhances Innate Immune Responses via Derepressing RELA Expression. MBio. 2019;10: pubmed publisher
Yin Q, Hu S, Xu Y, Yang L, Carmichael G, Chen L. SnoVectors for nuclear expression of RNA. Nucleic Acids Res. 2015;43:e5 pubmed publisher

product information

Catalog Number :

73174

Product Name :

pZW1-snoVector

article :

doi	10.1093/nar/gku1050
id	16117
pubmed_id	25378317

bacterial resistance :

Kanamycin

cloning :

backbone

pZW1

backbone_mutation

backbone_origin

Zefeng Wang's lab

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Two snoRNA genes SNORD116-13 and SNORD116-14 from the Prader Willi syndrome deletion region flanked by multiple cloning sites were inserted into the weak intron of Enhanced Green Fluoresence Protein (EGFP) such that only proper splicing leads to EGFP fluorescence.

growth strain :

Nuclear expression of RNAs

origin :

pi :

alt_names

HBII-85-13

cloning

clone_method	Restriction Enzyme
cloning_site_3	SacII
cloning_site_5	EcoRI
promoter	CMV promoter
sequencing_primer_3
sequencing_primer_5	EGFP-Seq-5F: GACCACATGAAGCAGCACGA
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	HBII-85-13
gene	SNORD116-13
id	100033425

genbank_ids

100033425

mutation

name

SNORD116-13

shRNA_sequence

size

species

9606

Homo sapiens