Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo | Addgene 73164 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

catalog :

73164

citations: 2

Reference
Rey G, Milev N, Valekunja U, Ch R, Ray S, Silva Dos Santos M, et al. Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes. Mol Syst Biol. 2018;14:e8376 pubmed publisher
Zielke N, Korzelius J, van Straaten M, Bender K, Schuhknecht G, Dutta D, et al. Fly-FUCCI: A versatile tool for studying cell proliferation in complex tissues. Cell Rep. 2014;7:588-598 pubmed publisher

product information

Catalog Number :

73164

Product Name :

Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

article :

doi	10.1016/j.celrep.2014.03.020
id	16476
pubmed_id	24726363

bacterial resistance :

Ampicillin

cloning :

backbone

Ac5-STABLE2-neo

backbone_mutation

backbone_origin

(Plasmid #32426)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth strain :

multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	EBVrev
sequencing_primer_5	AC5_PRIMER
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG6376, CG6376, DRTF1/E2F, DmE2F-1, Dmel CG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729
gene	E2f1
id	42550

aliases	Dmel_CG3510, 2g, 3510, CG3510, CYC-B, CYCB, CYCLIN B, Cyc B, Cycb, Cyclin B1, DmcycB, Dmel CG3510, chr2R:18312808. 18312907, cyc B, cyc-B, cycB, cycb, cyclin B, cyclinB, cyclinB1, mAbF2F4
gene	CycB
id	37618

genbank_ids

mutation

Cyclin B Fragment aa 1-266 & E2F1 Fragment aa 1-230

name

mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR

shRNA_sequence

size

3924

species

7227

Drosophila melanogaster