This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-hGal3
catalog :
73080
citations: 23
Reference
Jeethy Ram T, Lekshmi A, Darvin P, Rajappan P, Jagathnath Krishna K, Anoop T, et al. Co-expression of galectin-3 and vimentin in triple negative breast cancer cells promotes tumor progression, metastasis and survival. Tumour Biol. 2023;45:31-54 pubmed publisher
Torrino S, Oldham W, Tejedor A, Burgos I, Rachedi N, Fraissard K, et al. Mechano-dependent sorbitol accumulation supports biomolecular condensate. bioRxiv. 2023;: pubmed publisher
Soha S, Santhireswaran A, Huq S, Casimir Powell J, Jenkins N, Hodgson G, et al. Improved imaging and preservation of lysosome dynamics using silver nanoparticle-enhanced fluorescence. Mol Biol Cell. 2023;:mbcE22060200 pubmed publisher
Corkery D, Castro Gonzalez S, Knyazeva A, Herzog L, Wu Y. An ATG12-ATG5-TECPR1 E3-like complex regulates unconventional LC3 lipidation at damaged lysosomes. EMBO Rep. 2023;:e56841 pubmed publisher
Bhardwaj M, Lee J, Versace A, Harper S, Goldman A, Crissey M, et al. Lysosomal lipid peroxidation regulates tumor immunity. J Clin Invest. 2023;133: pubmed publisher
Jing Y, Kobayashi M, Vu H, Kasahara A, Chen X, Pham L, et al. Therapeutic advantage of targeting lysosomal membrane integrity supported by lysophagy in malignant glioma. Cancer Sci. 2022;113:2716-2726 pubmed publisher
Rudolph M, Carsten A, Kulnik S, Aepfelbacher M, Wolters M. Live imaging of Yersinia translocon formation and immune recognition in host cells. PLoS Pathog. 2022;18:e1010251 pubmed publisher
Mathew M, Abramowitz L, Donaldson J, Hanover J. Nutrient-responsive O-GlcNAcylation dynamically modulates the secretion of glycan-binding protein galectin 3. J Biol Chem. 2022;298:101743 pubmed publisher
Bandyopadhyay U, Todorova P, Pavlova N, Tada Y, Thompson C, Finley L, et al. Leucine retention in lysosomes is regulated by starvation. Proc Natl Acad Sci U S A. 2022;119: pubmed publisher
Liu K, Kong L, Graham D, Carey K, Xavier R. SAC1 regulates autophagosomal phosphatidylinositol-4-phosphate for xenophagy-directed bacterial clearance. Cell Rep. 2021;36:109434 pubmed publisher
Nakatsuka A, Yamaguchi S, Eguchi J, Kakuta S, Iwakura Y, Sugiyama H, et al. A Vaspin-HSPA1L complex protects proximal tubular cells from organelle stress in diabetic kidney disease. Commun Biol. 2021;4:373 pubmed publisher
Gupta S, Yano J, Mercier V, Htwe H, Shin H, Rademaker G, et al. Lysosomal retargeting of Myoferlin mitigates membrane stress to enable pancreatic cancer growth. Nat Cell Biol. 2021;23:232-242 pubmed publisher
Bonet Ponce L, Beilina A, Williamson C, Lindberg E, Kluss J, Saez Atienzar S, et al. LRRK2 mediates tubulation and vesicle sorting from lysosomes. Sci Adv. 2020;6: pubmed publisher
Zou X, Meng F, Fu C, Zhou J, Zhang Y, Wang R, et al. LZ-106, a potent lysosomotropic agent, causing TFEB-dependent cytoplasmic vacuolization. Gene. 2020;760:145017 pubmed publisher
Herbst S, Campbell P, Harvey J, Bernard E, Papayannopoulos V, Wood N, et al. LRRK2 activation controls the repair of damaged endomembranes in macrophages. EMBO J. 2020;:e104494 pubmed publisher
Chen J, Nathaniel D, Raghavan P, Nelson M, Tian R, Tse E, et al. Compromised function of the ESCRT pathway promotes endolysosomal escape of tau seeds and propagation of tau aggregation. J Biol Chem. 2019;294:18952-18966 pubmed publisher
Radhi O, Davidson S, Scott F, Zeng R, Jones D, Tomkinson N, et al. Inhibition of the ULK1 protein complex suppresses Staphylococcus-induced autophagy and cell death. J Biol Chem. 2019;294:14289-14307 pubmed publisher
Mathew M, Donaldson J. Distinct cargo-specific response landscapes underpin the complex and nuanced role of galectin-glycan interactions in clathrin-independent endocytosis. J Biol Chem. 2018;293:7222-7237 pubmed publisher
Gaidt M, Ebert T, Chauhan D, Ramshorn K, Pinci F, Zuber S, et al. The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3. Cell. 2017;171:1110-1124.e18 pubmed publisher
Gallagher L, Radhi O, Abdullah M, McCluskey A, Boyd M, Chan E. Lysosomotropism depends on glucose: a chloroquine resistance mechanism. Cell Death Dis. 2017;8:e3014 pubmed publisher
Stewart S, Menzies S, Popa S, Savinykh N, Petrunkina Harrison A, Lehner P, et al. A genome-wide CRISPR screen reconciles the role of N-linked glycosylation in galectin-3 transport to the cell surface. J Cell Sci. 2017;130:3234-3247 pubmed publisher
Otomo T, Yoshimori T. Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy-Dependent Recovery. Methods Mol Biol. 2017;1594:141-149 pubmed publisher
Maejima I, Takahashi A, Omori H, Kimura T, Takabatake Y, Saitoh T, et al. Autophagy sequesters damaged lysosomes to control lysosomal biogenesis and kidney injury. EMBO J. 2013;32:2336-47 pubmed publisher
product information
Catalog Number :
73080
Product Name :
pEGFP-hGal3
article :
doi10.1038/emboj.2013.171
id9941
pubmed_id23921551
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutationNo major modification to backbone. Digested with Bgl1+Sal1 to add the insert cDNA.
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid is used to detect ruptured lysosomal membrane by lysosomotropic agents or endosomal membrane accompanied with invading bacteria.
growth strain :
Expression in mammalian cells
origin :
37
pi :
alt_names
LGALS3
cloning
clone_methodRestriction Enzyme
cloning_site_3Sal1
cloning_site_5Bgl2
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2
geneLGALS3
id3958
genbank_ids
mutation
nameGalectin-3
shRNA_sequence
size754
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEGFP
resistance markers :
603
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA