pRR-AR | Addgene 73045 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRR-AR

catalog :

73045

citations: 1

Reference
Mazumder M, McMillen D. Design and characterization of a dual-mode promoter with activation and repression capability for tuning gene expression in yeast. Nucleic Acids Res. 2014;42:9514-22 pubmed publisher

product information

Catalog Number :

73045

Product Name :

pRR-AR

article :

doi	10.1093/nar/gku651
id	10372
pubmed_id	25056312

bacterial resistance :

Ampicillin

cloning :

backbone

pRW95-3

backbone_mutation

backbone_origin

PMID: 8800671

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

Please note that there are several discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function. This plasmid is a non-integrative plasmid that maintains approximately a single copy per cell and was used to express the glyceraldehyde phosphate dehydrogenase (GPD) driven androgen receptor gene. pRR-AR-5Z, a gift from Charles Miller (Addgene plasmid # 23058) was modified by excising the 5RE-cyc and lacZ portions using the BlpI and AatII restriction enzymes to create pRR-AR.

growth strain :

Constitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	glyceraldehyde phosphate dehydrogenase (GPD)
sequencing_primer_3
sequencing_primer_5	GPDpro (CGGTAGGTATTGATTGTAATTCTG)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
gene	AR
id	367

genbank_ids

mutation

please see depositor comments below

name

Androgen receptor

shRNA_sequence

size

species

9606

Homo sapiens