This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28-Mff(1-61)-PP-GST
catalog :
73042
citations: 2
Reference
Wang L, Xu Y, Yun S, Yuan Q, Satpute Krishnan P, Ye Y. SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum. Cell Rep. 2023;42:112028 pubmed publisher
Liu R, Chan D. The mitochondrial fission receptor Mff selectively recruits oligomerized Drp1. Mol Biol Cell. 2015;26:4466-77 pubmed publisher
product information
Catalog Number :
73042
Product Name :
pET28-Mff(1-61)-PP-GST
article :
doi10.1091/mbc.E15-08-0591
id16432
pubmed_id26446846
bacterial resistance :
Kanamycin
cloning :
backbonepET28a(+)
backbone_mutationA pET21b promoter (BglII/BamHI), followed by a PreScission protease site (BamHI/HindIII) and the GST sequence from pGEX6P1 (NotI/XhoI, Amersham) replaces the region between BglII/XhoI of pET28a(+)
backbone_originEMD Millipore (Novagen)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site
origin :
37
pi :
alt_names
mitochondrial fission factor
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoterT7
sequencing_primer_3CTAGTTATTGCTCAGCGGTG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliases5230400G24Rik
geneMff
id75734
genbank_ids
mutationTruncation containing amino acids 1-61
nameMff(1-61)
shRNA_sequence
size
species
10090
Mus musculus
tags
locationC terminal on backbone
tagPreScission protease site
locationC terminal on backbone
tagGST-tag
resistance markers :
665
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA