This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLV-NT-3/D15A
catalog :
73035
citations: 1
Reference
Enomoto M, Bunge M, Tsoulfas P. A multifunctional neurotrophin with reduced affinity to p75NTR enhances transplanted Schwann cell survival and axon growth after spinal cord injury. Exp Neurol. 2013;248:170-82 pubmed publisher
product information
Catalog Number :
73035
Product Name :
pLV-NT-3/D15A
article :
doi10.1016/j.expneurol.2013.06.013
id9995
pubmed_id23792206
bacterial resistance :
Ampicillin
cloning :
backbonepRRLsinPPT.CMV.MCS.Wpre
backbone_mutation
backbone_originNaldini
backbone_size6700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells
growth temp :
Grow on low salt, 5gm, of LB
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3none
cloning_site_5XbaI and BamHI
promoter
sequencing_primer_3
sequencing_primer_5cmv_frw_primer
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesHDNF, NGF-2, NGF2, NT-3, NT3
geneNTF3
id4908
genbank_ids
mutationMutated Aspartic acid in position 15 of the mature NT-3 to alanine. With this mutation NT-3 can bind also TrkB.
nameNT-3/D15A
shRNA_sequence
size800
species
9606
Homo sapiens
tags
resistance markers :
1214
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA