This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
N1-mSin1.1-GFP
catalog :
72907
citations: 1
Reference
Ebner M, Sinkovics B, Szczygieł M, Ribeiro D, Yudushkin I. Localization of mTORC2 activity inside cells. J Cell Biol. 2017;216:343-353 pubmed publisher
product information
Catalog Number :
72907
Product Name :
N1-mSin1.1-GFP
article :
doi10.1083/jcb.201610060
id25053
pubmed_id28143890
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutationKozak and start ATG before eGFP were mutated: caccatgg to aaccttgg Linker was added before Kozak (aaccttgg): cgggcccgggatccaccggtcgc
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
mSin1.1 was amplified from HEK293 cDNA library
growth strain :
GFP-tagged mSin1 (isoform 1)
origin :
37
pi :
alt_names
MAPKAP1
stress-activated map kinase interacting protein 1
cloning
clone_methodRestriction Enzyme
cloning_site_3SacII
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3tccagctcgaccaggatgg
sequencing_primer_5taacaactccgccccatt
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesJC310, MIP1, SIN1, SIN1b, SIN1g
geneMAPKAP1
id79109
genbank_ids
mutation
namemSin1.1
shRNA_sequence
size1566
species
9606
Homo sapiens
tags
locationC terminal on insert
tageGFP
resistance markers :
3233
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA