This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PMXS-NDI1
catalog :
72876
citations: 14
Reference
Chen X, Haribowo A, Baik A, Fossati A, Stevenson E, Chen Y, et al. In vivo protein turnover rates in varying oxygen tensions nominate MYBBP1A as a mediator of the hyperoxia response. Sci Adv. 2023;9:eadj4884 pubmed publisher
Bezwada D, Lesner N, Brooks B, Vu H, Wu Z, Cai L, et al. Mitochondrial metabolism in primary and metastatic human kidney cancers. bioRxiv. 2023;: pubmed publisher
Pachnis P, Wu Z, Faubert B, Tasdogan A, Gu W, Shelton S, et al. In vivo isotope tracing reveals a requirement for the electron transport chain in glucose and glutamine metabolism by tumors. Sci Adv. 2022;8:eabn9550 pubmed publisher
Zhu X, Guo Y, Chu C, Liu D, Duan K, Yin Y, et al. BRN2 as a key gene drives the early primate telencephalon development. Sci Adv. 2022;8:eabl7263 pubmed publisher
Donati G, Ravà M, Filipuzzi M, Nicoli P, Cassina L, Verrecchia A, et al. Targeting mitochondrial respiration and the BCL2 family in high-grade MYC-associated B-cell lymphoma. Mol Oncol. 2021;: pubmed publisher
Tramonti A, Cuyàs E, Encinar J, Pietzke M, Paone A, Verdura S, et al. Metformin Is a Pyridoxal-5'-phosphate (PLP)-Competitive Inhibitor of SHMT2. Cancers (Basel). 2021;13: pubmed publisher
Schwartz A, Goyert J, Solanki S, Kerk S, Chen B, Castillo C, et al. Hepcidin sequesters iron to sustain nucleotide metabolism and mitochondrial function in colorectal cancer epithelial cells. Nat Metab. 2021;3:969-982 pubmed publisher
Momcilovic M, Jones A, Bailey S, Waldmann C, Li R, Lee J, et al. In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer. Nature. 2019;575:380-384 pubmed publisher
Lin S, Huang C, Sun J, Bollt O, Wang X, Martine E, et al. The mitochondrial deoxyguanosine kinase is required for cancer cell stemness in lung adenocarcinoma. EMBO Mol Med. 2019;11:e10849 pubmed publisher
Vaughan R, Rothbart S, Dickson B. The finger loop of the SRA domain in the E3 ligase UHRF1 is a regulator of ubiquitin targeting and is required for the maintenance of DNA methylation. J Biol Chem. 2019;294:15724-15732 pubmed publisher
Shum M, Houde V, Bellemare V, Moreira R, Bellmann K, St Pierre P, et al. Inhibition of mitochondrial complex 1 by the S6K1 inhibitor PF-4708671 partly contributes to its glucose metabolic effects in muscle and liver cells. J Biol Chem. 2019;: pubmed publisher
Scott A, Walker S, Krank J, Wilkinson A, Johnson K, Lewis E, et al. AIF promotes a JNK1-mediated cadherin switch independently of respiratory chain stabilization. J Biol Chem. 2018;293:14707-14722 pubmed publisher
Zhu X, Zhou W, Jin H, Li T. Brn2 Alone Is Sufficient to Convert Astrocytes into Neural Progenitors and Neurons. Stem Cells Dev. 2018;27:736-744 pubmed publisher
Birsoy K, Possemato R, Lorbeer F, Bayraktar E, Thiru P, Yücel B, et al. Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides. Nature. 2014;508:108-12 pubmed publisher
product information
Catalog Number :
72876
Product Name :
PMXS-NDI1
article :
doi10.1038/nature13110
id16421
pubmed_id24670634
bacterial resistance :
Ampicillin
cloning :
backbonepMXs-IRES-blasticidin
backbone_mutation
backbone_origin
backbone_size5639
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The retroviral NDI1 vector was generated by cloning into the EcoRI and XhoI sites of the pMXS-ires-blast vector a cDNA insert generated by PCR using the primers below, followed by standard cloning techniques. Ndi1 EcoRI F: ATGAATTCCATCACATCATCGAATTAC Ndi1 XhoI R: ATCTCGAGAAAAGGGCATGTTAATTTCATCTATAAT It is a retroviral plasmid so it may be recommended to be propagated at 30oc but author typically grows it at 37oC.
growth strain :
expresses NDI1 in mammalian cells
origin :
30
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Xho1
cloning_site_5EcoR1
promoter
sequencing_primer_3
sequencing_primer_5pLXSN 5' CCCTTGAACCTCCTCGTTCGACC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYML120C
geneNDI1
id854919
genbank_ids
854919
NM_001182483.1
mutation
nameNDI1
shRNA_sequence
size1542
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
432
tags :
Unknown
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA