PMXS-SLC1A3 | Addgene 72873 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PMXS-SLC1A3

catalog :

72873

citations: 6

Reference
Wu S, Fukumoto T, Lin J, Nacarelli T, Wang Y, Ong D, et al. Targeting glutamine dependence through GLS1 inhibition suppresses ARID1A-inactivated clear cell ovarian carcinoma. Nat Cancer. 2021;2:189-200 pubmed publisher
Madala H, Helenius I, Zhou W, Mills E, Zhang Y, Liu Y, et al. Nitrogen Trapping as a Therapeutic Strategy in Tumors with Mitochondrial Dysfunction. Cancer Res. 2020;80:3492-3506 pubmed publisher
Cohen E, Geck R, Toker A. Metabolic pathway alterations in microvascular endothelial cells in response to hypoxia. PLoS ONE. 2020;15:e0232072 pubmed publisher
Bertero T, Oldham W, Grasset E, Bourget I, Boulter E, Pisano S, et al. Tumor-Stroma Mechanics Coordinate Amino Acid Availability to Sustain Tumor Growth and Malignancy. Cell Metab. 2019;29:124-140.e10 pubmed publisher
Sullivan L, Luengo A, Danai L, Bush L, Diehl F, Hosios A, et al. Aspartate is an endogenous metabolic limitation for tumour growth. Nat Cell Biol. 2018;20:782-788 pubmed publisher
Birsoy K, Wang T, Chen W, Freinkman E, Abu Remaileh M, Sabatini D. An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis. Cell. 2015;162:540-51 pubmed publisher

product information

Catalog Number :

72873

Product Name :

PMXS-SLC1A3

article :

doi	10.1016/j.cell.2015.07.016
id	16420
pubmed_id	26232224

bacterial resistance :

Ampicillin

cloning :

backbone

pMXs-IRES-blasticidin

backbone_mutation

backbone_origin

backbone_size

5639

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

It is a retroviral plasmid so it may be recommended to be propagated at 30 C but author typically grows it at 37 C.

growth strain :

The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly

origin :

pi :

EAAT1, GLAST, GLAST1geneSLC1A3id6507genbank_ids

NM_004172.4

mutationnameSLC1A3shRNA_sequencesize1629species

9606

Homo sapiens

tags
Cloningcloning_site_3
cloning_site
_5promotertd>sequencing_pri
mer_3sequenc
ing_primer_5GCCGGATCTAGCTAGTTA
ATTAAGccaccATGACTAAAAGCAATGGAGAAGAGCCC<
/td>site_3_destroyed<
td>site_5_destroyedtd>
entrez_gene

aliases

EA6,

alt_names

EA6; EAAT1; GLAST; GLAST1

cloning

clone_method

Gibson

resistance markers :

432

tags :

Unknown

terms :

Blasticidin