PMXS-GOT1 | Addgene 72872 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PMXS-GOT1

catalog :

72872

citations: 2

Reference
Madala H, Helenius I, Zhou W, Mills E, Zhang Y, Liu Y, et al. Nitrogen Trapping as a Therapeutic Strategy in Tumors with Mitochondrial Dysfunction. Cancer Res. 2020;80:3492-3506 pubmed publisher
Birsoy K, Wang T, Chen W, Freinkman E, Abu Remaileh M, Sabatini D. An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis. Cell. 2015;162:540-51 pubmed publisher

product information

Catalog Number :

72872

Product Name :

PMXS-GOT1

article :

doi	10.1016/j.cell.2015.07.016
id	16420
pubmed_id	26232224

bacterial resistance :

Ampicillin

cloning :

backbone

pMXs-IRES-blasticidin

backbone_mutation

backbone_origin

backbone_size

5639

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

It is a retroviral plasmid so it may be recommended to be propagated at 30oc but author typically grows it at 37oC.

growth strain :

The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.

origin :

pi :

alt_names

AST1; cCAT; GIG18; cAspAT; ASTQTL1

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	GCCGGATCTAGCTAGTTAATTAAG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AST, AST1, ASTQTL1, GIG18, SGOT, cAspAT, cCAT
gene	GOT1
id	2805

genbank_ids

NM_002079.2

mutation

sgRNA resistant human GOT1, 7 silent mutations c138t, t141c, c144a, c657t,c660a,c663t,c669t

name

GOT1

shRNA_sequence

size

1242

species

9606

Homo sapiens