This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe HA- Raf1(S642A):ER* puro
catalog :
72572
citations: 1
Reference
Jensen K, Moyer C, Janes K. Network Architecture Predisposes an Enzyme to Either Pharmacologic or Genetic Targeting. Cell Syst. 2016;2:112-121 pubmed
product information
Catalog Number :
72572
Product Name :
pBabe HA- Raf1(S642A):ER* puro
article :
doi10.1016/j.cels.2016.01.012
id18888
pubmed_id26942229
bacterial resistance :
Ampicillin
cloning :
backbonepBabe ER* puro
backbone_mutation
backbone_originMartin McMahon
backbone_size6000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
Expresses hormone-responsive, feedback-resistant Raf allele with an N-terminal HA tag.
origin :
37
pi :
alt_names
CRAF
Raf-1
c-Raf
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5pBabe 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMD1NN, CRAF, NS5, Raf-1, c-Raf
geneRAF1
id5894
genbank_ids
NM_002880
mutationamino acids 306-648, serine 642 mutated to alanine
nameRaf1
shRNA_sequence
size2037
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
locationC terminal on backbone
tagER*
plasmid copy :
Deborah Morrison
resistance markers :
1585
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA