This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGLS3-5xHRE-p53(K120R)
catalog :
72554
citations: 1
Reference
Leszczynska K, Foskolou I, Abraham A, Anbalagan S, Tellier C, Haider S, et al. Hypoxia-induced p53 modulates both apoptosis and radiosensitivity via AKT. J Clin Invest. 2015;125:2385-98 pubmed publisher
product information
Catalog Number :
72554
Product Name :
pGLS3-5xHRE-p53(K120R)
article :
doi10.1172/JCI80402
id16357
pubmed_id25961455
bacterial resistance :
Ampicillin
cloning :
backbonepGL3-Basic
backbone_mutationp53 was cloned so that it replaced luciferase. Neo cassette was inserted into BamHI site.
backbone_originPromega
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5KpnI
promoterE1B minimal promoter
sequencing_primer_3pGLS3 R: 5 -gtatcttatcatgtctgctcg-3
sequencing_primer_5E1B F: 5 -agatctggtaggcgtgtacgg-3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutationK120R
namep53
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tag5XHRE
resistance markers :
3256
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA