This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJK642
catalog :
72391
citations: 1
Reference
McMullan G, Naydenova K, Mihaylov D, Yamashita K, Peet M, Wilson H, et al. Structure determination by cryoEM at 100 keV. Proc Natl Acad Sci U S A. 2023;120:e2312905120 pubmed publisher
product information
Catalog Number :
72391
Product Name :
pJK642
article :
doi
id16335
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepET28a
backbone_mutation
backbone_origin
backbone_size5369
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
origin :
37
pi :
alt_names
glnA
JW3841
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7 terminator
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb3870, ECK3863, JW3841
geneglnA
id948370
genbank_ids
mutationMutated tyrosine-397 to phenylalanine to block adenylylation.
nameglutamine synthetase
shRNA_sequence
size
species
32644
Other
Escherichia coli str. K-12 substr. MG1655)
tags
locationN terminal on backbone
tagHis6
plasmid copy :
Gene was amplified from a plasmid template provided by Jamie Williamson (Scripps Research Institute) and cloned by our lab.
resistance markers :
2599
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA