This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFETCh_ZNF219
catalog :
72367
product information
Catalog Number :
72367
Product Name :
pFETCh_ZNF219
article :
doi
id16333
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepFETCh_Donor
backbone_mutation
backbone_origin
backbone_size5771
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth notes :
Addgene's quality control sequencing finds a G to E amino acid residue substitution in the linker between ZNF219 and the 3xFlag. This residue change is not thought to affect plasmid function. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
growth strain :
Donor vector for 3' FLAG tag of human ZNF219
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3Neo-R
sequencing_primer_5Neo-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesZFP219
geneZNF219
id51222
genbank_ids
mutation
nameZNF219 Homology Arms
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tag3XFLAG-P2A-NeoR
resistance markers :
1728
2438
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA