This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Sema3d(S)-Fc-His
catalog :
72144
citations: 1
Reference
Visser J, Cheng Y, Perry S, Chastain A, Parsa B, Masri S, et al. An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina. elife. 2015;4:e08149 pubmed publisher
product information
Catalog Number :
72144
Product Name :
Sema3d(S)-Fc-His
article :
doi10.7554/eLife.08149
id16305
pubmed_id26633812
bacterial resistance :
Ampicillin
cloning :
backbonepCMVi-SV40ori
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Addgene Sanger sequencing found A126T within the Sema3d translation compared to NM_028882.4.
growth strain :
Expresses the Sema3D protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
origin :
37
pi :
alt_names
Semaphorin 3D
NCBI gene ID 108151; Reference sequence NM_028882.4
cloning
clone_methodRestriction Enzyme
cloning_site_3AscI
cloning_site_5NotI
promoterCMV
sequencing_primer_3ATCATGAGGGTGTCCTTG
sequencing_primer_5GTCAGAGGTAACTCCCGTTGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases4631426B19Rik
geneSema3d
id108151
genbank_ids
mutation
nameSema3d
shRNA_sequence
size1698
species
10090
Mus musculus
tags
locationC terminal on backbone
tagFc-His
resistance markers :
2566
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA