This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Unc5a.a-AP-His
catalog :
72050
citations: 1
Reference
Visser J, Cheng Y, Perry S, Chastain A, Parsa B, Masri S, et al. An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina. elife. 2015;4:e08149 pubmed publisher
product information
Catalog Number :
72050
Product Name :
Unc5a.a-AP-His
article :
doi10.7554/eLife.08149
id16305
pubmed_id26633812
bacterial resistance :
Ampicillin
cloning :
backbonepCMVi-SV40ori
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
We identified a previously-unreported isoform and named it z . As such, we designated this previously-reported isoform as isoform a .
growth strain :
Expresses the extracellular region of the Unc5A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.
origin :
37
pi :
alt_names
Unc5A, isoform a; Unc5h1
NCBI gene ID 107448; Reference sequence NM_153131.3
cloning
clone_methodRestriction Enzyme
cloning_site_3SpeI
cloning_site_5NotI
promoterCMV
sequencing_primer_3GAGGTTCTTGGCGGCTGTC
sequencing_primer_5GTCAGAGGTAACTCCCGTTGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesUnc5h1, mKIAA1976
geneUnc5a
id107448
genbank_ids
mutation
nameUnc5a.a
shRNA_sequence
size1062
species
10090
Mus musculus
tags
locationC terminal on backbone
tagAP-His
resistance markers :
2566
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA