pET-Sac-Abeta(M1-42) | Addgene 71875 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET-Sac-Abeta(M1-42)

catalog :

71875

citations: 6

Reference
Sun C, Slade L, Mbonu P, Ordner H, Mitchell C, Mitchell M, et al. Membrane protein chaperone and sodium chloride modulate the kinetics and morphology of amyloid beta aggregation. FEBS J. 2024;291:158-176 pubmed publisher
Gupta A, Lentzsch A, Siegel A, Yu Z, Chio U, Cheng Y, et al. Dodecamer assembly of a metazoan AAA+ chaperone couples substrate extraction to refolding. Sci Adv. 2023;9:eadf5336 pubmed publisher
Ayala Mariscal S, Pigazzini M, Richter Y, xd6 zel M, Grothaus I, Protze J, et al. Identification of a HTT-specific binding motif in DNAJB1 essential for suppression and disaggregation of HTT. Nat Commun. 2022;13:4692 pubmed publisher
Ferdosh S, Banerjee S, Singh J, Barat C. Amyloid protein-induced sequestration of the eukaryotic ribosome: effect of stoichiometry and polyphenolic inhibitors. FEBS Lett. 2022;596:1190-1202 pubmed publisher
Yoo S, Zhang S, Kreutzer A, Nowick J. An Efficient Method for the Expression and Purification of Aβ(M1-42). Biochemistry. 2018;57:3861-3866 pubmed publisher
Walsh D, Thulin E, Minogue A, Gustavsson N, Pang E, Teplow D, et al. A facile method for expression and purification of the Alzheimer's disease-associated amyloid beta-peptide. FEBS J. 2009;276:1266-81 pubmed publisher

product information

Catalog Number :

71875

Product Name :

pET-Sac-Abeta(M1-42)

article :

doi	10.1111/j.1742-4658.2008.06862.x
id	16259
pubmed_id	19175671

bacterial resistance :

Ampicillin

cloning :

backbone

pET vector

backbone_mutation

Vector is a modified pET vector. Restriction digest sites for Abeta gene are Nde1 (5') and Sac1 (3')

backbone_origin

unknown

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

origin :

pi :

alt_names

amyloid-beta 42

abeta 42

cloning

clone_method	Restriction Enzyme
cloning_site_3	Sac1
cloning_site_5	Nde1
promoter	T7
sequencing_primer_3	5'- GTG GGT TCT AAC AAG GGT GCT ATC ATC GGT CTG ATG GTT GGT GGC GTT GTG -3'
sequencing_primer_5	5'- ATG GAC GCT GAA TTC CGT CAC GAC TCT GGT TAC GAA GTT CAC CAC CAG AAG CTG GTG -3'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4
gene	APP
id	351

genbank_ids

mutation

name

Abeta(M1-42)

shRNA_sequence

size

129

species

9606

Homo sapiens