This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGFP-Kif5c
catalog :
71853
citations: 2
Reference
Chen X, Das U, Park G, Mobley W. Normal levels of KIF5 but reduced KLC1 levels in both Alzheimer disease and Alzheimer disease in Down syndrome: evidence suggesting defects in anterograde transport. Alzheimers Res Ther. 2021;13:59 pubmed publisher
Dunn S, Morrison E, Liverpool T, Molina París C, Cross R, Alonso M, et al. Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules in live cells. J Cell Sci. 2008;121:1085-95 pubmed publisher
product information
Catalog Number :
71853
Product Name :
pGFP-Kif5c
article :
doi10.1242/jcs.026492
id16255
pubmed_id18334549
bacterial resistance :
Kanamycin
cloning :
backbonepEGFPC3
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Following transfection, look at cells that have low levels of expression (for the wild type Kif5c protein) to investigate dynamic behaviour, as high levels of expression result in bundling of microtubules.
growth strain :
mammalian expression of GFP-Kif5c
origin :
37
pi :
alt_names
kinesin family member 5C
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneKif5c
id311024
genbank_ids
mutationKif5c starts at AA3
nameKif5c
shRNA_sequence
size3000
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagEGFP
resistance markers :
2662
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA