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product summary
company name :
Addgene
product type :
cDNA
product name :
pdCas9-DNMT3A-PuroR_hNT
catalog :
71830
citations: 1
product information
Catalog Number :
71830
Product Name :
pdCas9-DNMT3A-PuroR_hNT
article :
| doi | 10.1093/nar/gkw159 |
| id | 18013 |
| pubmed_id | 26969735 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pdCas9-DNMT3A-PuroR | ||
| backbone_mutation | |||
| backbone_origin | |||
| backbone_size | 10134 | ||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
The catalytic domain of human DNMT3A (amino acids P602-V912) was derived from the plasmid pcDNA3/Myc-DNMT3A (Addgene, plasmid #35521) (Chen et al., 2005, J Cell Biochem 95: 902-917). Undesired BbsI restriction site was removed by site-directed mutagenesis, without affecting the amino acid sequence. Plasmid pSpCas9n(BB)-2A-Puro (PX462) (Addgene, plasmid #48141) (Ran et al., 2013, Nat Protoc 8: 2281-2308) was used as a backbone. Additional H840A mutation was introduced into Cas9n D10A nickase. Human non-targeting sgRNA is cloned between BbsI restriction sites.
growth strain :
Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells
origin :
37
pi :
|
plasmid copy :
The plasmid is derived from Addgene plasmids #35521 and #48141.
resistance markers :
| 2660 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments