This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUAST-GFP-RpL10Ab
catalog :
71729
citations: 1
Reference
Thomas A, Lee P, Dalton J, Nomie K, Stoica L, Costa Mattioli M, et al. A versatile method for cell-specific profiling of translated mRNAs in Drosophila. PLoS ONE. 2012;7:e40276 pubmed publisher
product information
Catalog Number :
71729
Product Name :
pUAST-GFP-RpL10Ab
article :
doi10.1371/journal.pone.0040276
id16211
pubmed_id22792260
bacterial resistance :
Ampicillin
cloning :
backbonepUAST
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
The 5 ' restriction site used for cloning is NotI (EcoRI, BglII are 5' to that site) and there is an internal XhoI site at bp 84 of the 654 bp RpL10Ab fragment. There is a unique KpnI site immediately following the XhoI site at the 3' end of the cloned fragment. The NotI site and BglII sites are also usable for anyone wanting to reclone the fragment and the XhoI site is not usable but the KpnI site is.
growth strain :
Expression of Drosophila melanogaster Rpl10Ab-GFP fusion
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5NotI
promoterhsp70
sequencing_primer_3SV40pA-R
sequencing_primer_5pCasper-hs
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG7283, BcDNA:RH06366, CG7283, Dmel CG7283, L10Ab, RpL10A, RpL10Ae, anon-EST:Posey188
geneRpL10Ab
id39338
genbank_ids
mutation
nameRpL10Ab
shRNA_sequence
size
species
7227
Drosophila melanogaster
tags
locationN terminal on insert
tagEGFP
resistance markers :
2649
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA