This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)
catalog :
71720
citations: 9
Reference
Ho J, Guerrero L, Libuda D, Luxton G, Starr D. Actin and CDC-42 contribute to nuclear migration through constricted spaces in C. elegans. Development. 2023;150: pubmed publisher
Smith H, Lanjuin A, Sharma A, Prabhakar A, Nowak E, Stine P, et al. Neuronal mTORC1 inhibition promotes longevity without suppressing anabolic growth and reproduction in C. elegans. PLoS Genet. 2023;19:e1010938 pubmed publisher
Negishi T, Kitagawa S, Horii N, Tanaka Y, Haruta N, Sugimoto A, et al. The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans. Genetics. 2021;: pubmed publisher
Kurashina M, Wang J, Lin J, Lee K, Johal A, Mizumoto K. Sustained expression of unc-4 homeobox gene and unc-37/Groucho in postmitotic neurons specifies the spatial organization of the cholinergic synapses in C. elegans. elife. 2021;10: pubmed publisher
Ho J, Valdez V, Ma L, Starr D. Characterizing Dynein's Role in P-cell Nuclear Migration using an Auxin-Induced Degradation System. MicroPubl Biol. 2018;2018: pubmed publisher
Wirshing A, Cram E. Spectrin regulates cell contractility through production and maintenance of actin bundles in the Caenorhabditis elegans spermatheca. Mol Biol Cell. 2018;29:2433-2449 pubmed publisher
Kasimatis K, Moerdyk Schauwecker M, Phillips P. Auxin-Mediated Sterility Induction System for Longevity and Mating Studies in Caenorhabditis elegans. G3 (Bethesda). 2018;8:2655-2662 pubmed publisher
Liu P, Chen B, Mailler R, Wang Z. Antidromic-rectifying gap junctions amplify chemical transmission at functionally mixed electrical-chemical synapses. Nat Commun. 2017;8:14818 pubmed publisher
Zhang L, Ward J, Cheng Z, Dernburg A. The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans. Development. 2015;142:4374-84 pubmed publisher
product information
Catalog Number :
71720
Product Name :
pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)
article :
doi10.1242/dev.129635
id16207
pubmed_id26552885
bacterial resistance :
Ampicillin
cloning :
backbonepCFJ151
backbone_mutation
backbone_origin
backbone_size7373
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
growth notes :
Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
growth strain :
Expressing an mRuby-tagged TIR1 in the soma of C. elegans
origin :
37
pi :
alt_names
TIR1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promotereef-1A.1 (eft-3)
sequencing_primer_3
sequencing_primer_5ATGCAAAAGAGAATCGCCTTGTCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT3G62980, AtTIR1, TRANSPORT INHIBITOR RESPONSE 1
geneTIR1
id825473
genbank_ids
mutationcodon-optimized for expression in C. elegans; Two point mutations D170E and M473L incorporated
nameTRANSPORT INHIBITOR RESPONSE 1
shRNA_sequence
size1884
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tagmRuby
resistance markers :
2648
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA