This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
catalog :
71719
citations: 3
Reference
Smith H, Lanjuin A, Sharma A, Prabhakar A, Nowak E, Stine P, et al. Neuronal mTORC1 inhibition promotes longevity without suppressing anabolic growth and reproduction in C. elegans. PLoS Genet. 2023;19:e1010938 pubmed publisher
Edwards S, Erdenebat P, Morphis A, Kumar L, Wang L, Chamera T, et al. Insulin/IGF-1 signaling and heat stress differentially regulate HSF1 activities in germline development. Cell Rep. 2021;36:109623 pubmed publisher
Zhang L, Ward J, Cheng Z, Dernburg A. The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans. Development. 2015;142:4374-84 pubmed publisher
product information
Catalog Number :
71719
Product Name :
pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
article :
doi10.1242/dev.129635
id16207
pubmed_id26552885
bacterial resistance :
Ampicillin
cloning :
backbonepCFJ151
backbone_mutation
backbone_origin
backbone_size7373
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
growth notes :
Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
growth strain :
Expressing a GFP-tagged degron in the soma of C. elegans
origin :
37
pi :
alt_names
IAA17
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promotereef-1A.1 (eft-3)
sequencing_primer_3
sequencing_primer_5ATGCCTAAAGATCCAGCCAAACCTCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G04250, AUXIN RESISTANT 3, AtIAA17, F19P19.31, F19P19_31, IAA17, indole-3-acetic acid inducible 17
geneAXR3
id839568
genbank_ids
mutationminimal degron sequence (71-114aa) with start codon
nameauxin-responsive protein IAA17
shRNA_sequence
size135
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tagEmGFP
resistance markers :
2648
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA