This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pdCas9-DNMT3A-EGFP
catalog :
71666
citations: 20
Reference
Alvizi L, Nani D, Brito L, Kobayashi G, Passos Bueno M, Mayor R. Neural crest E-cadherin loss drives cleft lip/palate by epigenetic modulation via pro-inflammatory gene-environment interaction. Nat Commun. 2023;14:2868 pubmed publisher
Kehayova Y, Wilkinson J, Rice S, Loughlin J. Independent osteoarthritis risk-conferring alleles mediate the same epigenetic and transcriptional effect on a shared target gene, COLGALT2. Arthritis Rheumatol. 2022;: pubmed publisher
Singh K, Rustagi Y, Abouhashem A, Tabasum S, Verma P, Hernandez E, et al. Genome-wide DNA hypermethylation opposes healing in patients with chronic wounds by impairing epithelial-mesenchymal transition. J Clin Invest. 2022;132: pubmed publisher
Koike Y, Sugai A, Hara N, Ito J, Yokoseki A, Ishihara T, et al. Age-related demethylation of the TDP-43 autoregulatory region in the human motor cortex. Commun Biol. 2021;4:1107 pubmed publisher
Vizoso M, van Rheenen J. Diverse transcriptional regulation and functional effects revealed by CRISPR/Cas9-directed epigenetic editing. Oncotarget. 2021;12:1651-1662 pubmed publisher
Kehayova Y, Watson E, Wilkinson J, Loughlin J, Rice S. Genetic and Epigenetic Interplay Within a COLGALT2 Enhancer Associated With Osteoarthritis. Arthritis Rheumatol. 2021;73:1856-1865 pubmed publisher
Li M, Jiang P, Cheng K, Zhang Z, Lan S, Li X, et al. Regulation of MYB by distal enhancer elements in human myeloid leukemia. Cell Death Dis. 2021;12:223 pubmed publisher
Dahlet T, Argüeso Lleida A, Al Adhami H, Dumas M, Bender A, Ngondo R, et al. Genome-wide analysis in the mouse embryo reveals the importance of DNA methylation for transcription integrity. Nat Commun. 2020;11:3153 pubmed publisher
Deng M, Liu Z, Chen B, Cai Y, Wan Y, Wang F. Locus-Specific Regulation of Xist Expression Using the CRISPR-Cas9-Based System. DNA Cell Biol. 2020;39:572-578 pubmed publisher
Deutschmeyer V, Breuer J, Walesch S, Sokol A, Graumann J, Bartkuhn M, et al. Epigenetic therapy of novel tumour suppressor ZAR1 and its cancer biomarker function. Clin Epigenetics. 2019;11:182 pubmed publisher
Tarjan D, Flavahan W, Bernstein B. Epigenome editing strategies for the functional annotation of CTCF insulators. Nat Commun. 2019;10:4258 pubmed publisher
Liu J, Banerjee A, Herring C, Attalla J, Hu R, Xu Y, et al. Neurog3-Independent Methylation Is the Earliest Detectable Mark Distinguishing Pancreatic Progenitor Identity. Dev Cell. 2019;48:49-63.e7 pubmed publisher
Kantor B, Tagliafierro L, Gu J, Zamora M, Ilich E, Grenier C, et al. Downregulation of SNCA Expression by Targeted Editing of DNA Methylation: A Potential Strategy for Precision Therapy in PD. Mol Ther. 2018;26:2638-2649 pubmed publisher
Marx N, Grünwald Gruber C, Bydlinski N, Dhiman H, Ngoc Nguyen L, Klanert G, et al. CRISPR-Based Targeted Epigenetic Editing Enables Gene Expression Modulation of the Silenced Beta-Galactoside Alpha-2,6-Sialyltransferase 1 in CHO Cells. Biotechnol J. 2018;13:e1700217 pubmed publisher
Qian P, De Kumar B, He X, Nolte C, Gogol M, Ahn Y, et al. Retinoid-Sensitive Epigenetic Regulation of the Hoxb Cluster Maintains Normal Hematopoiesis and Inhibits Leukemogenesis. Cell Stem Cell. 2018;22:740-754.e7 pubmed publisher
Galonska C, Charlton J, Mattei A, Donaghey J, Clement K, Gu H, et al. Genome-wide tracking of dCas9-methyltransferase footprints. Nat Commun. 2018;9:597 pubmed publisher
Huang Y, Su J, Lei Y, Brunetti L, Gundry M, Zhang X, et al. DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A. Genome Biol. 2017;18:176 pubmed publisher
Xiong T, Meister G, Workman R, Kato N, Spellberg M, Turker F, et al. Targeted DNA methylation in human cells using engineered dCas9-methyltransferases. Sci Rep. 2017;7:6732 pubmed publisher
Lei Y, Zhang X, Su J, Jeong M, Gundry M, Huang Y, et al. Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein. Nat Commun. 2017;8:16026 pubmed publisher
Vojta A, Dobrinić P, Tadić V, Bočkor L, Korać P, Julg B, et al. Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Nucleic Acids Res. 2016;44:5615-28 pubmed publisher
product information
Catalog Number :
71666
Product Name :
pdCas9-DNMT3A-EGFP
article :
doi10.1093/nar/gkw159
id18013
pubmed_id26969735
bacterial resistance :
Ampicillin
cloning :
backbonePX462
backbone_mutation
backbone_origin
backbone_size4246
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
The catalytic domain of human DNMT3A (amino acids P602-V912) was derived from the plasmid pcDNA3/Myc-DNMT3A (Addgene, plasmid #35521) (Chen et al., 2005, J Cell Biochem 95: 902-917). Undesired BbsI restriction site was removed by site-directed mutagenesis, without affecting the amino acid sequence. Plasmid pSpCas9n(BB)-2A-Puro (PX462) (Addgene, plasmid #48141) (Ran et al., 2013, Nat Protoc 8: 2281-2308) was used as a backbone. Additional H840A mutation was introduced into Cas9n D10A nickase. T2A-PuroR EcoRI fragment was replaced with T2A-EGFP fragment from pSpCas9n(BB)-2A-GFP (PX461) (Addgene, plasmid #48140) (Ran et al., 2013, Nat Protoc 8: 2281-2308).
growth strain :
Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
origin :
37
pi :
alt_names
dCas9-DNMT3A-EGFP
SpdCas9-DNMT3A-T2A-EGFP
dCas9-DNMT3A
cloning
clone_methodRestriction Enzyme
cloning_site_3FseI
cloning_site_5BamHI
promoterCBh
sequencing_primer_3BGH Rev
sequencing_primer_5GS-linker For 5' GAAGAGGTACACCAGCACCAAAG 3'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDNMT3A2, HESJAS, M.HsaIIIA, TBRS
geneDNMT3A
id1788
genbank_ids
AKA60242.1
NP_072046.2
mutationD10A and H840A in S.pyogenes Cas9
nameS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP
shRNA_sequence
size6020
species
9606
Homo sapiens
100
Synthetic
32644
Other
S. pyogenes
tags
locationN terminal on insert
tag3xFLAG
locationN terminal on insert
tagSV40 NLS
locationC terminal on insert
tagT2A-EGFP
plasmid copy :
The plasmid is derived from Addgene plasmids #35521, #48141 and #48140.
resistance markers :
2660
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA