This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET151-hUCH37 (ISF1)(M148D/F149D)
catalog :
71388
citations: 1
Reference
Vander Linden R, Hemmis C, Schmitt B, Ndoja A, Whitby F, Robinson H, et al. Structural basis for the activation and inhibition of the UCH37 deubiquitylase. Mol Cell. 2015;57:901-911 pubmed publisher
product information
Catalog Number :
71388
Product Name :
pET151-hUCH37 (ISF1)(M148D/F149D)
article :
doi10.1016/j.molcel.2015.01.016
id9834
pubmed_id25702872
bacterial resistance :
Ampicillin
cloning :
backbonepET151-D-TOPO
backbone_mutation
backbone_originInvotrogen
backbone_size5800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
M148D/F149D mutations were incorporated via Quickchange mutagenesis.
growth strain :
Expression of hUCH37 (M148D/F149D) in E.coli
origin :
37
pi :
alt_names
UCHL5
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCGI-70, INO80R, UCH-L5, UCH37
geneUCHL5
id51377
genbank_ids
AAD31528.1
AAD31528.1
mutationchanged methionine 148 to aspartate, phenylalanine 149 to aspartate
namehUCH37 (ISF1) M148D/F149D
shRNA_sequence
size989
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHIS-TEV sequence
resistance markers :
2231
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA