This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
csd6_1-330/pET28b(+) N-tag
catalog :
71350
citations: 1
Reference
Kim H, Im H, An D, Yoon J, Jang J, Mobashery S, et al. The Cell Shape-determining Csd6 Protein from Helicobacter pylori Constitutes a New Family of L,D-Carboxypeptidase. J Biol Chem. 2015;290:25103-17 pubmed publisher
product information
Catalog Number :
71350
Product Name :
csd6_1-330/pET28b(+) N-tag
article :
doi10.1074/jbc.M115.658781
id16072
pubmed_id26306031
bacterial resistance :
Kanamycin
cloning :
backbonepET28(+)
backbone_mutation
backbone_originNovagen
backbone_size5300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
csd6, construct:1-330, pET28b(+) N-tag
growth temp :
To produce protein, transform into Rosetta2(DE3)pLysS cells. induction: 30 degree @ 20 hours
inserts :
DH5alpha
origin :
37
pi :
alt_names
Csd6
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7 terminator
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHP0518
geneHP0518
id900044
genbank_ids
mutation
nameHP0518
shRNA_sequence
size990
species
tags
locationN terminal on backbone
tagHistidine tag
resistance markers :
508
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA