This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSMART-Ala2
catalog :
71326
citations: 1
Reference
Ye Z, Li S, Hennigan J, Lebeau J, Moreb E, Wolf J, et al. Two-stage dynamic deregulation of metabolism improves process robustness & scalability in engineered E. coli. Metab Eng. 2021;68:106-118 pubmed publisher
product information
Catalog Number :
71326
Product Name :
pSMART-Ala2
article :
doi10.1016/j.ymben.2021.09.009
id28224721
pubmed_id34600151
bacterial resistance :
Kanamycin
cloning :
backbonepSMART-HCKan (AF532107.1)
backbone_mutation
backbone_originLucigen (Middleton, WI)
backbone_size1800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2020.08.30.274290v1.full.
growth strain :
NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression
origin :
37
pi :
alt_names
AlaDH
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterinsulated waaHp from E. coli
sequencing_primer_3GGT CAG GTA TGA TTT AA A TGG TCA GT
sequencing_primer_5CAG TCC AGT TAC GCT GGA GTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBSU31930
geneald
id936557
genbank_ids
mutationD196A, L197R
nameNADPH Alanine Dehydrogenase
shRNA_sequence
size1200
species
100
Synthetic
tags
resistance markers :
2382
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA