pBABEpuro-HA-p62-LIR | Addgene 71306 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBABEpuro-HA-p62-LIR

catalog :

71306

citations: 3

Reference
Cui Y, Yang S, Wei J, Shea C, Zhong W, Wang F, et al. Autophagy of the m6A mRNA demethylase FTO is impaired by low-level arsenic exposure to promote tumorigenesis. Nat Commun. 2021;12:2183 pubmed publisher
Panda P, Naik P, Meher B, Das D, Mukhopadhyay S, Praharaj P, et al. PUMA dependent mitophagy by Abrus agglutinin contributes to apoptosis through ceramide generation. Biochim Biophys Acta Mol Cell Res. 2018;1865:480-495 pubmed publisher
Chen N, Eritja N, Lock R, Debnath J. Autophagy restricts proliferation driven by oncogenic phosphatidylinositol 3-kinase in three-dimensional culture. Oncogene. 2013;32:2543-54 pubmed publisher

product information

Catalog Number :

71306

Product Name :

pBABEpuro-HA-p62-LIR

article :

doi	10.1038/onc.2012.277
id	16057
pubmed_id	22777351

bacterial resistance :

Ampicillin

cloning :

backbone

pBABE-puro

backbone_mutation

backbone_origin

Addgene plasmid # 1764

backbone_size

5169

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

This plasmid contains a T375A mutation within the Sequestosome-1 translation compared to NP_003891.1. The depositor states that this mutation should not affect plasmid function.

growth strain :

Retroviral expression of p62 with W338A point mutation

origin :

pi :

alt_names

p62

SQSTM1

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	pBABE 3'
sequencing_primer_5	pBABE 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	A170, DMRV, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B
gene	SQSTM1
id	8878

genbank_ids

mutation

Mutated Tryptophan at position 338 to Alanine (W338A)

name

Sequestosome-1

shRNA_sequence

size

species

9606

Homo sapiens