This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABEpuro-HA-p62
catalog :
71305
citations: 1
Reference
Chen N, Eritja N, Lock R, Debnath J. Autophagy restricts proliferation driven by oncogenic phosphatidylinositol 3-kinase in three-dimensional culture. Oncogene. 2013;32:2543-54 pubmed publisher
product information
Catalog Number :
71305
Product Name :
pBABEpuro-HA-p62
article :
doi10.1038/onc.2012.277
id16057
pubmed_id22777351
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-puro
backbone_mutation
backbone_originAddgene plasmid # 1764
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
This plasmid contains a T375A mutation within the Sequestosome-1 translation compared to NP_003891.1. The depositor states that this mutation does not affect plasmid function.
growth strain :
Retroviral expression of p62
origin :
37
pi :
alt_names
p62
SQSTM1
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3pBABE 3'
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesA170, DMRV, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B
geneSQSTM1
id8878
genbank_ids
mutation
nameSequestosome-1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
plasmid copy :
Human p62 cDNA was a kind gift from Dr. T. Johansen.
resistance markers :
636
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA